Research Papers: Sensing

Detection of biothiols in cells by a terbium chelate-Hg (II) system

[+] Author Affiliations
Hongliang Tan, Yang Chen

Southeast University, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjing 210096, China

J. Biomed. Opt. 17(1), 017001 (Jan 19, 2012). doi:10.1117/1.JBO.17.1.017001
History: Received July 27, 2011; Revised October 28, 2011; Accepted November 2, 2011
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Abstract.  Great efforts have been devoted to the development of sensitive and specific analysis methods for biothiols because of their important roles in biological systems. We present a new detection system for biothiols that is based on the reversible quenching and restoration of fluorescence of terbium chelate caused by Hg2+ and thiol species. In the presence of biothiols, a restoration of fluorescence of terbium chelate after quenching by Hg2+ was observed due to the interaction of Hg2+ with thiol groups, and the restored fluorescence increased with the concentration of biothiols. This method was sensitive and selective for biothiols. The detection limit was 80 nM for glutathione, 100 nM for Hcy, and 400 nM for Cysteine, respectively. The terbium chelate-Hg (II) system was successfully applied to determine the levels of biothiols in cancer cells and urine samples. Further, it was also shown to be comparable to Ellman’s assay. Compared to other fluorescence methods, the terbium chelate probe is advantageous because interference from short-lived nonspecific fluorescence can be efficiently eliminated due to the long fluorescence lifetime of terbium chelate, which allows for detection by time-resolved fluorescence. The terbium chelate probe can serve as a diagnostic tool for the detection of abnormal levels of biothiols in disease.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Hongliang Tan and Yang Chen
"Detection of biothiols in cells by a terbium chelate-Hg (II) system", J. Biomed. Opt. 17(1), 017001 (Jan 19, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.1.017001


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