Research Papers: Sensing

Magnetic field enriched surface enhanced resonance Raman spectroscopy for early malaria diagnosis

[+] Author Affiliations
Clement Yuen, Quan Liu

Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, Singapore 637457

J. Biomed. Opt. 17(1), 017005 (Feb 07, 2012). doi:10.1117/1.JBO.17.1.017005
History: Received August 27, 2011; Revised November 18, 2011; Accepted November 30, 2011
Text Size: A A A

Abstract.  Hemozoin is a by-product of malaria infection in erythrocytes, which has been explored as a biomarker for early malaria diagnosis. We report magnetic field-enriched surface-enhanced resonance Raman spectroscopy (SERRS) of β—hematin crystals, which are the equivalent of hemozoin biocrystals in spectroscopic features, by using magnetic nanoparticles with iron oxide core and silver shell (Fe3O4@Ag). The external magnetic field enriches β—hematin crystals and enhances the binding between β—hematin crystals and magnetic nanoparticles, which provides further improvement in SERRS signals. The magnetic field-enriched SERRS signal of β—hematin crystals shows approximately five orders of magnitude enhancement in the resonance Raman signal, in comparison to about three orders of magnitude improvement in the SERRS signal without the influence of magnetic field. The improvement has led to a β—hematin detection limit at a concentration of 5 nM (roughly equivalent to 30 parasites/μl at the early stages of malaria infection), which demonstrates the potential of magnetic field-enriched SERRS technique in early malaria diagnosis.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Clement Yuen and Quan Liu
"Magnetic field enriched surface enhanced resonance Raman spectroscopy for early malaria diagnosis", J. Biomed. Opt. 17(1), 017005 (Feb 07, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.1.017005


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.