Research Papers: Sensing

Identifying compositional and structural changes in spongy and subchondral bone from the hip joints of patients with osteoarthritis using Raman spectroscopy

[+] Author Affiliations
Tomasz Buchwald, Marek Kozielski, Mirosław Szybowicz

Poznan University of Technology, Faculty of Technical Physics, Nieszawska 13a, 60-965 Poznan, Poland

Krzysztof Niciejewski, Hanna Krauss

Poznan University of Medical Sciences, Faculty of Medicine I, Swiecickiego 6, 60-784 Poznan, Poland

Marcin Siatkowski

DZNE, German Center for Neurodegenerative Disorders, Gehlsheimer Strasse 20, D-18147 Rostock, Germany

University of Rostock, Institute for Biostatistics and Informatics in Medicine and Ageing Research, Ernst Heydemann-Strasse 8, D-18057 Rostock, Germany

J. Biomed. Opt. 17(1), 017007 (Jan 31, 2012). doi:10.1117/1.JBO.17.1.017007
History: Received August 22, 2011; Revised November 9, 2011; Accepted November 30, 2011
Text Size: A A A

Abstract.  Raman microspectroscopy was used to examine the biochemical composition and molecular structure of extracellular matrix in spongy and subchondral bone collected from patients with clinical and radiological evidence of idiopathic osteoarthritis of the hip and from patients who underwent a femoral neck fracture, as a result of trauma, without previous clinical and radiological evidence of osteoarthritis. The objectives of the study were to determine the levels of mineralization, carbonate accumulation and collagen quality in bone tissue. The subchondral bone from osteoarthritis patients in comparison with control subject is less mineralized due to a decrease in the hydroxyapatite concentration. However, the extent of carbonate accumulation in the apatite crystal lattice increases, most likely due to deficient mineralization. The alpha helix to random coil band area ratio reveals that collagen matrix in subchondral bone is more ordered in osteoarthritis disease. The hydroxyapatite to collagen, carbonate apatite to hydroxyapatite and alpha helix to random coil band area ratios are not significantly changed in the differently loaded sites of femoral head. The significant differences also are not visible in mineral and organic constituents’ content in spongy bone beneath the subchondral bone in osteoarthritis disease.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Tomasz Buchwald ; Krzysztof Niciejewski ; Marek Kozielski ; Mirosław Szybowicz ; Marcin Siatkowski, et al.
"Identifying compositional and structural changes in spongy and subchondral bone from the hip joints of patients with osteoarthritis using Raman spectroscopy", J. Biomed. Opt. 17(1), 017007 (Jan 31, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.1.017007


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.