Research Papers: Imaging

Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

[+] Author Affiliations
Moonseok Kim, Youngwoon Choi, Wonshik Choi

Korea University, Department of Physics, Seoul 136-701, Republic of Korea

Christopher Fang-Yen

University of Pennsylvania, Department of Bioengineering, Philadelphia, Pennsylvania 19104

Yongjin Sung, Ramachandra R. Dasari, Michael S. Feld

Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, Cambridge, Massachusetts 02139

Kwanhyung Kim

LG Electronics Inc., Seoul 137-130, Republic of Korea

J. Biomed. Opt. 17(2), 026003 (Feb 23, 2012). doi:10.1117/1.JBO.17.2.026003
History: Received June 6, 2011; Revised December 6, 2011; Accepted December 7, 2011
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Abstract.  We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Moonseok Kim ; Youngwoon Choi ; Christopher Fang-Yen ; Yongjin Sung ; Kwanhyung Kim, et al.
"Three-dimensional differential interference contrast microscopy using synthetic aperture imaging", J. Biomed. Opt. 17(2), 026003 (Feb 23, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.2.026003


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