Research Papers: Imaging

Polarization-interferometry-based wavelength-interrogation surface plasmon resonance imager for analysis of microarrays

[+] Author Affiliations
Zhiyi Liu, Heng Shi, Xinyuan Chong, Suihua Ma, Jihua Guo, Hui Ma, Yonghong He

Tsinghua University, Graduate School at Shenzhen, Laboratory of Optical Imaging and Sensing, Shenzhen, 518055, China

Le Liu

Tsinghua University, Graduate School at Shenzhen, Laboratory of Advanced Power Source, Shenzhen, 518055, China

Xiaoxiao Wang

Tsinghua University, School of Life Science, Beijing, 100084, China

Yanhong Ji

South China Normal University, MOE Key laboratory of Laser Life Science & Institute of Laser Life Science, Guangzhou, 510631, China

J. Biomed. Opt. 17(3), 036002 (Mar 12, 2012). doi:10.1117/1.JBO.17.3.036002
History: Received May 8, 2011; Revised January 3, 2012; Accepted January 6, 2012
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Abstract.  Polarization interferometry (PI) techniques, which are able to improve surface plasmon resonance (SPR) sensing performance and reduce restrictions on allowable parameters of SPR-supporting metal films, have been experimentally realized only in SPR sensors using monochromatic light as a source. Wavelength-interrogation SPR sensors modulated by PI techniques have not been reported due to the wavelength-sensitive characterization of PI phase compensators. In this work we develop a specially designed rhombic prism for phase compensating which is totally insensitive to wavelength. For the first time we successfully apply PI technique to a wavelength-interrogation SPR imager. This imager is able to offer two-dimensional imaging of the whole array plane. As a result of PI modulation, resolutions of 1.3×106 refractive index unit (RIU) under the normal condition and 3.9×107 RIU under a more time-consuming condition are acquired. The application of this imager was demonstrated by reading microarrays for identification of bacteria, and SPR results were confirmed by means of fluorescence imaging.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Zhiyi Liu ; Le Liu ; Xiaoxiao Wang ; Heng Shi ; Xinyuan Chong, et al.
"Polarization-interferometry-based wavelength-interrogation surface plasmon resonance imager for analysis of microarrays", J. Biomed. Opt. 17(3), 036002 (Mar 12, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.3.036002


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