Research Papers: Imaging

Autofocus evaluation for brightfield microscopy pathology

[+] Author Affiliations
Rafael Redondo

Instituto de Óptica, Consejo Superior de Investigaciones Científicas (CSIC), Serrano 121, Madrid 28006, Spain

Universidad de Castilla-La Mancha, VISILAB, Camilo José Cela 3, Ciudad Real 13071, Spain

Gloria Bueno, Oscar Déniz, Jesus Salido, Maria del Milagro Fernández, Juan Vidal

Universidad de Castilla-La Mancha, VISILAB, Camilo José Cela 3, Ciudad Real 13071, Spain

Juan Carlos Valdiviezo

Instituto Nacional de Astrofísica, Óptica y Electrónica (INAOE), Tonantzintla, Puebla, Mexico

Rodrigo Nava, Boris Escalante-Ramírez

Universidad Nacional Autónoma de México, Facultad de Ingeniería, Mexico City, Mexico

Gabriel Cristóbal

Instituto de Óptica, Consejo Superior de Investigaciones Científicas (CSIC), Serrano 121, Madrid 28006, Spain

Marcial García-Rojo

Hospital General Universitario de Ciudad Real, Obispo Rafael Torija, s/n, Ciudad Real 13005, Spain

J. Biomed. Opt. 17(3), 036008 (Mar 26, 2012). doi:10.1117/1.JBO.17.3.036008
History: Received September 28, 2011; Revised January 15, 2012; Accepted January 19, 2012
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Abstract.  An essential and indispensable component of automated microscopy framework is the automatic focusing system, which determines the in-focus position of a given field of view by searching the maximum value of a focusing function over a range of z-axis positions. The focus function and its computation time are crucial to the accuracy and efficiency of the system. Sixteen focusing algorithms were analyzed for histological and histopathological images. In terms of accuracy, results have shown an overall high performance by most of the methods. However, we included in the evaluation study other criteria such as computational cost and focusing curve shape which are crucial for real-time applications and were used to highlight the best practices.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Rafael Redondo ; Gloria Bueno ; Juan Carlos Valdiviezo ; Rodrigo Nava ; Gabriel Cristóbal, et al.
"Autofocus evaluation for brightfield microscopy pathology", J. Biomed. Opt. 17(3), 036008 (Mar 26, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.3.036008


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