Research Papers: Therapeutic

Development of a new illumination procedure for photodynamic therapy of the abdominal cavity

[+] Author Affiliations
Laurie Guyon

University Lille Nord de France, INSERM, U703, Lille University Hospital, Lille, France

Lille University Hospital, Department of Gynaecology and Obstetrics, Lille, France

Jean Claude Lesage, Nacim Betrouni

Lille University Hospital, Department of Gynaecology and Obstetrics, Lille, France

Serge Mordon

Lille University Hospital, Department of Gynaecology and Obstetrics, Lille, France

GDR 3049, Médicaments Photoactivables—Photochimiothérapie (PHOTOMED)

J. Biomed. Opt. 17(3), 038001 (Mar 08, 2012). doi:10.1117/1.JBO.17.3.038001
History: Received September 30, 2011; Revised December 20, 2011; Accepted January 11, 2012
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Abstract.  A homogeneous illumination of intra-abdominal organs is essential for successful photodynamic therapy of the abdominal cavity. Considering the current lack of outstanding light-delivery systems, a new illumination procedure was assessed. A rat model of peritoneal carcinomatosis was used. Four hours after intraperitoneal injection of hexaminolevulinate, a square illuminating panel connected to a 635-nm laser source was inserted vertically into the abdominal cavity. The abdominal incision was sutured and a pneumoperitoneum created prior to illumination. Light dosimetry was based on the calculation of the peritoneal surface by MRI. The rats were treated with a light dose of 20, 10, 5 or 2.5J/cm2 administered continuously with an irradiance of 7mW/cm2. The homogeneity of the cavity illumination was assessed by quantification of the photobleaching of the tumor lesions according to their localization and by scoring of that of the liver and of the bowel immediately after treatment. Photobleaching quantification for tumor lesions relied on the calculation of the fluorescence intensity ratio (after/before treatment) after recording of the lesions during blue-light laparoscopy and determination of their fluorescence intensity with Sigmascan Pro software. The procedure led to a homogeneous treatment of the abdominal cavity. No statistical difference was observed for the photobleaching values according to the localization of the lesions on the peritoneum (p=0.59) and photobleaching of the liver and of the intestine was homogeneous. We conclude that this procedure can successfully treat the major sites involved in peritoneal carcinomatosis.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Laurie Guyon ; Jean Claude Lesage ; Nacim Betrouni and Serge Mordon
"Development of a new illumination procedure for photodynamic therapy of the abdominal cavity", J. Biomed. Opt. 17(3), 038001 (Mar 08, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.3.038001


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