JBO Letters

Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics

[+] Author Affiliations
Martí Duocastella, Bo Sun, Craig B. Arnold

Princeton University, Department of Mechanical and Aerospace Engineering, Princeton, New Jersey 08544

J. Biomed. Opt. 17(5), 050505 (May 07, 2012). doi:10.1117/1.JBO.17.5.050505
History: Received February 25, 2012; Revised March 30, 2012; Accepted April 2, 2012
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Abstract.  Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Martí Duocastella ; Bo Sun and Craig B. Arnold
"Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics", J. Biomed. Opt. 17(5), 050505 (May 07, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.5.050505


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