Research Papers: Imaging

Optimal ultraviolet wavelength for in vivo photoacoustic imaging of cell nuclei

[+] Author Affiliations
Da-Kang Yao, Konstantin Maslov, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, St. Louis, Missouri 63130

Ruimin Chen, Qifa Zhou

University of Southern California, Department of Biomedical Engineering, Los Angeles, California 90089

J. Biomed. Opt. 17(5), 056004 (May 04, 2012). doi:10.1117/1.JBO.17.5.056004
History: Received December 5, 2011; Revised February 29, 2012; Accepted March 5, 2012
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Abstract.  In order to image noninvasively cell nuclei in vivo without staining, we have developed ultraviolet photoacoustic microscopy (UV-PAM), in which ultraviolet light excites nucleic acids in cell nuclei to produce photoacoustic waves. Equipped with a tunable laser system, the UV-PAM was applied to in vivo imaging of cell nuclei in small animals. We found that 250 nm was the optimal wavelength for in vivo photoacoustic imaging of cell nuclei. The optimal wavelength enables UV-PAM to image cell nuclei using as little as 2 nJ laser pulse energy. Besides the optimal wavelength, application of a wavelength between 245 and 275 nm can produce in vivo images of cell nuclei with specific, positive, and high optical contrast.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Da-Kang Yao ; Ruimin Chen ; Konstantin Maslov ; Qifa Zhou and Lihong V. Wang
"Optimal ultraviolet wavelength for in vivo photoacoustic imaging of cell nuclei", J. Biomed. Opt. 17(5), 056004 (May 04, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.5.056004


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