Research Papers: Imaging

Discrimination of motile bacteria from filamentous fungi using dynamic speckle

[+] Author Affiliations
Silvia E. Murialdo, J. Froilán Gonzalez

Universidad Nacional de Mar del Plata, Departamento de Ingeniería Química, Facultad de Ingeniería, Grupo de Ingeniería Bioquímica, Juan B. Justo 4302, 7600, Mar del Plata, Argentina

Lucía I. Passoni

Universidad Nacional de Mar del Plata, Facultad de Ingeniería, Laboratorio de Bioingeniería, Juan B. Justo 4302, 7600, Mar del Plata, Argentina

Marcelo N. Guzman, G. Hernán Sendra

Universidad Nacional de Mar del Plata, Facultad de Ingeniería, Laboratorio Láser, Juan B. Justo 4302, 7600, Mar del Plata, Argentina

Universidad Nacional de la Plata, Argentina, Departamento de Ciencias Básicas, Facultad de Ingeniería, Centro de Investigaciones Ópticas (CONICET La Plata-CIC), UID Optimo, P.O. Box 3, 1897, Gonnet, La Plata, Argentina

Héctor Rabal, Marcelo Trivi

Universidad Nacional de la Plata, Argentina, Departamento de Ciencias Básicas, Facultad de Ingeniería, Centro de Investigaciones Ópticas (CONICET La Plata-CIC), UID Optimo, P.O. Box 3, 1897, Gonnet, La Plata, Argentina

J. Biomed. Opt. 17(5), 056011 (May 07, 2012). doi:10.1117/1.JBO.17.5.056011
History: Received September 21, 2011; Revised March 29, 2012; Accepted March 30, 2012
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Abstract.  We present a dynamic laser speckle method to easily discriminate filamentous fungi from motile bacteria in soft surfaces, such as agar plate. The method allows the detection and discrimination between fungi and bacteria faster than with conventional techniques. The new procedure could be straightforwardly extended to different micro-organisms, as well as applied to biological and biomedical research, infected tissues analysis, and hospital water and wastewaters studies.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Silvia E. Murialdo ; Lucía I. Passoni ; Marcelo N. Guzman ; G. Hernán Sendra ; Héctor Rabal, et al.
"Discrimination of motile bacteria from filamentous fungi using dynamic speckle", J. Biomed. Opt. 17(5), 056011 (May 07, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.5.056011


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