Research Papers: Imaging

Fourier transform infrared spectroscopic imaging of cardiac tissue to detect collagen deposition after myocardial infarction

[+] Author Affiliations
Rabee Cheheltani, Jenna M. Rosano

Temple University, Department of Mechanical Engineering, Philadelphia, Pennsylvania

Bin Wang

Temple University, Department of Mechanical Engineering, Philadelphia, Pennsylvania

Widener University, Department of Biomedical Engineering, Chester, Pennsylvania

Abdel Karim Sabri

Temple University School of Medicine, Department of Physiology, Cardiovascular Research Center, Philadelphia, Pennsylvania

Nancy Pleshko

Temple University, Department of Bioengineering, Philadelphia, Pennsylvania

Mohammad F. Kiani

Temple University, Department of Mechanical Engineering, Philadelphia, Pennsylvania

Temple University School of Medicine, Department of Radiation Oncology, Philadelphia, Pennsylvania

J. Biomed. Opt. 17(5), 056014 (May 11, 2012). doi:10.1117/1.JBO.17.5.056014
History: Received December 13, 2011; Revised March 2, 2012; Accepted March 5, 2012
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Abstract.  Myocardial infarction often leads to an increase in deposition of fibrillar collagen. Detection and characterization of this cardiac fibrosis is of great interest to investigators and clinicians. Motivated by the significant limitations of conventional staining techniques to visualize collagen deposition in cardiac tissue sections, we have developed a Fourier transform infrared imaging spectroscopy (FT-IRIS) methodology for collagen assessment. The infrared absorbance band centered at 1338cm1, which arises from collagen amino acid side chain vibrations, was used to map collagen deposition across heart tissue sections of a rat model of myocardial infarction, and was compared to conventional staining techniques. Comparison of the size of the collagen scar in heart tissue sections as measured with this methodology and that of trichrome staining showed a strong correlation (R=0.93). A Pearson correlation model between local intensity values in FT-IRIS and immuno-histochemical staining of collagen type I also showed a strong correlation (R=0.86). We demonstrate that FT-IRIS methodology can be utilized to visualize cardiac collagen deposition. In addition, given that vibrational spectroscopic data on proteins reflect molecular features, it also has the potential to provide additional information about the molecular structure of cardiac extracellular matrix proteins and their alterations.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Topics

Collagen ; Tissues ; Heart

Citation

Rabee Cheheltani ; Jenna M. Rosano ; Bin Wang ; Abdel Karim Sabri ; Nancy Pleshko, et al.
"Fourier transform infrared spectroscopic imaging of cardiac tissue to detect collagen deposition after myocardial infarction", J. Biomed. Opt. 17(5), 056014 (May 11, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.5.056014


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