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Research Papers: Imaging

In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy

[+] Author Affiliations
Jan Laufer, Paul Beard

University College London, Department of Medical Physics and Bioengineering, Gower Street, London WC1E 6BT, United Kingdom

University College London, Centre for Advanced Biomedical Imaging, Department of Medicine and Institute of Child Health, Paul O’Gorman Building, 72 Huntley Street, London WC1E 6BT, United Kingdom

Peter Johnson, Barbara Pedley

University College London, Centre for Advanced Biomedical Imaging, Department of Medicine and Institute of Child Health, Paul O’Gorman Building, 72 Huntley Street, London WC1E 6BT, United Kingdom

University College London, The UCL Cancer Institute, Paul O’Gorman Building, 72 Huntley Street, London WC1E 6DD, United Kingdom

Edward Zhang, Bradley Treeby, Ben Cox

University College London, Department of Medical Physics and Bioengineering, Gower Street, London WC1E 6BT, United Kingdom

J. Biomed. Opt. 17(5), 056016 (May 21, 2012). doi:10.1117/1.JBO.17.5.056016
History: Received November 23, 2011; Revised February 28, 2012; Accepted March 2, 2012
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Abstract.  The use of a novel all-optical photoacoustic scanner for imaging the development of tumor vasculature and its response to a therapeutic vascular disrupting agent is described. The scanner employs a Fabry-Perot polymer film ultrasound sensor for mapping the photoacoustic waves and an image reconstruction algorithm based upon attenuation-compensated acoustic time reversal. The system was used to noninvasively image human colorectal tumor xenografts implanted subcutaneously in mice. Label-free three-dimensional in vivo images of whole tumors to depths of almost 10 mm with sub-100-micron spatial resolution were acquired in a longitudinal manner. This enabled the development of tumor-related vascular features, such as vessel tortuosity, feeding vessel recruitment, and necrosis to be visualized over time. The system was also used to study the temporal evolution of the response of the tumor vasculature following the administration of a therapeutic vascular disrupting agent (OXi4503). This revealed the well-known destruction and recovery phases associated with this agent. These studies illustrate the broader potential of this technology as an imaging tool for the preclinical and clinical study of tumors and other pathologies characterized by changes in the vasculature.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Jan Laufer ; Peter Johnson ; Edward Zhang ; Bradley Treeby ; Ben Cox, et al.
"In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy", J. Biomed. Opt. 17(5), 056016 (May 21, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.5.056016


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