Research Papers: Sensing

Light-scattering flow cytometry for identification and characterization of blood microparticles

[+] Author Affiliations
Anastasiya I. Konokhova, Maxim A. Yurkin, Alexander E. Moskalensky, Andrei V. Chernyshev, Valeri P. Maltsev

Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, 630090, Novosibirsk, Russia

Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia

Galina A. Tsvetovskaya

ANO “Center of New Medical Technologies in Akademgorodok”, Pirogova 25/4, 630090, Novosibirsk, Russia

Institute of Chemical Biology and Fundamental Medicine, Lavrentiev Avenue 8, 630090, Novosibirsk, Russia

Elena D. Chikova

Novosibirsk State University, Pirogova 2, 630090, Novosibirsk, Russia

ANO “Center of New Medical Technologies in Akademgorodok”, Pirogova 25/4, 630090, Novosibirsk, Russia

Institute of Chemical Biology and Fundamental Medicine, Lavrentiev Avenue 8, 630090, Novosibirsk, Russia

J. Biomed. Opt. 17(5), 057006 (May 10, 2012). doi:10.1117/1.JBO.17.5.057006
History: Received January 31, 2012; Revised March 29, 2012; Accepted March 29, 2012
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Abstract.  We describe a novel approach to study blood microparticles using the scanning flow cytometer, which measures light scattering patterns (LSPs) of individual particles. Starting from platelet-rich plasma, we separated spherical microparticles from non-spherical plasma constituents, such as platelets and cell debris, based on similarity of their LSP to that of sphere. This provides a label-free method for identification (detection) of microparticles, including those larger than 1 µm. Next, we rigorously characterized each measured particle, determining its size and refractive index including errors of these estimates. Finally, we employed a deconvolution algorithm to determine size and refractive index distributions of the whole population of microparticles, accounting for largely different reliability of individual measurements. Developed methods were tested on a blood sample of a healthy donor, resulting in good agreement with literature data. The only limitation of this approach is size detection limit, which is currently about 0.5 µm due to used laser wavelength of 0.66 µm.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Anastasiya I. Konokhova ; Maxim A. Yurkin ; Alexander E. Moskalensky ; Andrei V. Chernyshev ; Galina A. Tsvetovskaya, et al.
"Light-scattering flow cytometry for identification and characterization of blood microparticles", J. Biomed. Opt. 17(5), 057006 (May 10, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.5.057006


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