Research Papers: Imaging

Sensitivity analysis of near-infrared functional lymphatic imaging

[+] Author Affiliations
Michael Weiler, Timothy Kassis, J. Brandon Dixon

Georgia Institute of Technology, George W. Woodruff School of Mechanical Engineering, Wallace H. Coulter Department of Biomedical Engineering, Parker H. Petit Institute for Bioengineering and Bioscience, IBB 2312, 315 Ferst Drive, Atlanta, Georgia 30332-0405

J. Biomed. Opt. 17(6), 066019 (Jun 08, 2012). doi:10.1117/1.JBO.17.6.066019
History: Received January 30, 2012; Revised April 6, 2012; Accepted April 30, 2012
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Abstract.  Near-infrared imaging of lymphatic drainage of injected indocyanine green (ICG) has emerged as a new technology for clinical imaging of lymphatic architecture and quantification of vessel function, yet the imaging capabilities of this approach have yet to be quantitatively characterized. We seek to quantify its capabilities as a diagnostic tool for lymphatic disease. Imaging is performed in a tissue phantom for sensitivity analysis and in hairless rats for in vivo testing. To demonstrate the efficacy of this imaging approach to quantifying immediate functional changes in lymphatics, we investigate the effects of a topically applied nitric oxide (NO) donor glyceryl trinitrate ointment. Premixing ICG with albumin induces greater fluorescence intensity, with the ideal concentration being 150μg/mL ICG and 60g/L albumin. ICG fluorescence can be detected at a concentration of 150μg/mL as deep as 6 mm with our system, but spatial resolution deteriorates below 3 mm, skewing measurements of vessel geometry. NO treatment slows lymphatic transport, which is reflected in increased transport time, reduced packet frequency, reduced packet velocity, and reduced effective contraction length. NIR imaging may be an alternative to invasive procedures measuring lymphatic function in vivo in real time.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Michael Weiler ; Timothy Kassis and J. Brandon Dixon
"Sensitivity analysis of near-infrared functional lymphatic imaging", J. Biomed. Opt. 17(6), 066019 (Jun 08, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.6.066019


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