Figure 2 shows representative en-face and B-scan OCT images of isolated retina in which the retinal pigment epithelium (RPE) was not included. During the recording, the photoreceptors were upward, i.e., facing to the objective, to improve image quality of the outer retina. Given the sub-cellular resolution, the en-face image [Fig. 2(a)] revealed individual photoreceptors. B-scan image [Fig. 2(b)] disclosed individual layers from the photoreceptor to inner retina. After adapting band nomenclature used in high resolution OCT of human retina to frog retina,2 we labeled individual layers. As shown in Fig. 2(b), the OS, ‘IS/OS’ (presumed), OLM, OPL, inner plexiform layer (IPL), and nerve fiber layer (NFL) were hyper-reflective; while the outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL) were hypo-reflective. The bright ‘IS/OS’ band was bumpy in the high resolution OCT image. In order to achieve better visualization of individual OCT bands, Fig. 2(c) shows an averaged cross-section image over 120 μm (200 B-scan images, with 0.6 μm recording interval) along the -axis. We consistently observed that the whole OS section, not only the OS tip or the ‘IS/OS’ junction, produced OCT signal; and the band of lower reflectivity [red arrow in Fig. 2(c)] was observed located sclerally to the 2nd OCT band. Moreover, bright spots [red arrows in Fig. 2(a) and 2(b)], with dimension at cellular level, were consistently observed. This might reflect reflectance inhomogeneity of different photoreceptors in the frog retina that has two types each of cones and rods.