Special Section on Photoacoustic Imaging and Sensing

Capture of circulating tumor cells using photoacoustic flowmetry and two phase flow

[+] Author Affiliations
Christine M. O’Brien, Kyle D. Rood, Kiran Bhattacharyya, Thiago DeSouza, Sagar K. Gupta, Jeffrey D. Mosley, Benjamin S. Goldschmidt

University of Missouri, Department of Biological Engineering, Columbia, Missouri 65212

University of Missouri, Christopher S. Bond Life Sciences Center, Columbia, Missouri 65211

Shramik Sengupta

University of Missouri, Department of Biological Engineering, Columbia, Missouri 65212

Nikhilesh Sharma

University of Missouri, Christopher S. Bond Life Sciences Center, Columbia, Missouri 65211

John A. Viator

University of Missouri, Department of Biological Engineering, Columbia, Missouri 65212

University of Missouri, Christopher S. Bond Life Sciences Center, Columbia, Missouri 65211

University of Missouri, Department of Dermatology, Columbia, Missouri 65211

J. Biomed. Opt. 17(6), 061221 (May 07, 2012). doi:10.1117/1.JBO.17.6.061221
History: Received July 6, 2011; Revised February 27, 2012; Accepted March 13, 2012
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Abstract.  Melanoma is the deadliest form of skin cancer, yet current diagnostic methods are unable to detect early onset of metastatic disease. Patients must wait until macroscopic secondary tumors form before malignancy can be diagnosed and treatment prescribed. Detection of cells that have broken off the original tumor and travel through the blood or lymph system can provide data for diagnosing and monitoring metastatic disease. By irradiating enriched blood samples spiked with cultured melanoma cells with nanosecond duration laser light, we induced photoacoustic responses in the pigmented cells. Thus, we can detect and enumerate melanoma cells in blood samples to demonstrate a paradigm for a photoacoustic flow cytometer. Furthermore, we capture the melanoma cells using microfluidic two phase flow, a technique that separates a continuous flow into alternating microslugs of air and blood cell suspension. Each slug of blood cells is tested for the presence of melanoma. Slugs that are positive for melanoma, indicated by photoacoustic waves, are separated from the cytometer for further purification and isolation of the melanoma cell. In this paper, we evaluate the two phase photoacoustic flow cytometer for its ability to detect and capture metastastic melanoma cells in blood.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Christine M. O’Brien ; Kyle D. Rood ; Kiran Bhattacharyya ; Thiago DeSouza ; Shramik Sengupta, et al.
"Capture of circulating tumor cells using photoacoustic flowmetry and two phase flow", J. Biomed. Opt. 17(6), 061221 (May 07, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.6.061221


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