Special Section on Optical Diagnostic and Biophotonic Methods from Bench to Bedside

Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models

[+] Author Affiliations
Irina V. Larina, Saba H. Syed, Mary E. Dickinson

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030

Narendran Sudheendran

University of Houston, Department of Biomedical Engineering, N 207 Engineering Building 1, Houston, Texas 77204

Paul A. Overbeek

Baylor College of Medicine, Department of Molecular and Cellular Biology, One Baylor Plaza, Houston, Texas 77030

Kirill V. Larin

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030

University of Houston, Department of Biomedical Engineering, N 207 Engineering Building 1, Houston, Texas 77204

J. Biomed. Opt. 17(8), 081410 (May 14, 2012). doi:10.1117/1.JBO.17.8.081410
History: Received January 29, 2012; Revised April 16, 2012; Accepted April 18, 2012
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Abstract.  Mouse models of ocular diseases provide a powerful resource for exploration of molecular regulation of eye development and pre-clinical studies. Availability of a live high-resolution imaging method for mouse embryonic eyes would significantly enhance longitudinal analyses and high-throughput morphological screening. We demonstrate that optical coherence tomography (OCT) can be used for live embryonic ocular imaging throughout gestation. At all studied stages, the whole eye is within the imaging distance of the system and there is a good optical contrast between the structures. We also performed OCT eye imaging in the embryonic retinoblastoma mouse model Pax6-SV40 T-antigen, which spontaneously forms lens and retinal lesions, and demonstrate that OCT allows us to clearly differentiate between the mutant and wild type phenotypes. These results demonstrate that OCTin utero imaging is a potentially useful tool to study embryonic ocular diseases in mouse models.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Irina V. Larina ; Saba H. Syed ; Narendran Sudheendran ; Paul A. Overbeek ; Mary E. Dickinson, et al.
"Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models", J. Biomed. Opt. 17(8), 081410 (May 14, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.8.081410


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