Special Section on Laser Technologies for Biomedical Applications

Nanosurgery of cells and chromosomes using near-infrared twelve-femtosecond laser pulses

[+] Author Affiliations
Aisada Uchugonova, Karsten König

Saarland University, Department of Biophotonics and Laser Technology, Campus A5 1, 66123 Saarbruecken, Germany

Matthias Lessel, Christian Zeitz, Karin Jacobs

Saarland University, Experimental Physics, Campus E2 9, 66123 Saarbruecken, Germany

Sander Nietzsche

Jena University Hospital, Center of Electron Microscopy, Ziegenmühlenweg, 07743 Jena, Germany

Cornelius Lemke

Jena University Hospital, Institute of Anatomy I, Teichgraben 7, 07743 Jena, Germany

J. Biomed. Opt. 17(10), 101502 (May 16, 2012). doi:10.1117/1.JBO.17.10.101502
History: Received December 9, 2011; Revised March 1, 2012; Accepted March 2, 2012
Text Size: A A A

Abstract.  Laser-assisted surgery based on multiphoton absorption of near-infrared laser light has great potential for high precision surgery at various depths within the cells and tissues. Clinical applications include refractive surgery (fs-LASIK). The non-contact laser method also supports contamination-free cell nanosurgery. In this paper we describe usage of an ultrashort femtosecond laser scanning microscope for sub-100 nm surgery of human cells and metaphase chromosomes. A mode-locked 85 MHz Ti:Sapphire laser with an M-shaped ultrabroad band spectrum (maxima:770nm/830nm) and an in situ pulse duration at the target ranging from 12 fs up to 3 ps was employed. The effects of laser nanoprocessing in cells and chromosomes have been quantified by atomic force microscopy. These studies demonstrate the potential of extreme ultrashort femtosecond laser pulses at low mean milliwatt powers for sub-100 nm surgery of cells and cellular organelles.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Aisada Uchugonova ; Matthias Lessel ; Sander Nietzsche ; Christian Zeitz ; Karin Jacobs, et al.
"Nanosurgery of cells and chromosomes using near-infrared twelve-femtosecond laser pulses", J. Biomed. Opt. 17(10), 101502 (May 16, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.10.101502


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.