Special Section on Laser Technologies for Biomedical Applications

Cellular viscoelasticity probed by active rheology in optical tweezers

[+] Author Affiliations
Evgeny V. Lyubin, Maria D. Khokhlova, Maria N. Skryabina, Andrey A. Fedyanin

Lomonosov Moscow State University, Faculty of Physics, 119991 Moscow, Russia

J. Biomed. Opt. 17(10), 101510 (Jun 08, 2012). doi:10.1117/1.JBO.17.10.101510
History: Received February 21, 2012; Revised April 12, 2012; Accepted April 23, 2012
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Abstract.  A novel approach to probe viscoelastic properties of cells based on double trap optical tweezers is reported. Frequency dependence of the tangent of phase difference in the movement of the opposite erythrocyte edges while one of the edges is forced to oscillate by optical tweezers appeared to be highly dependent on the rigidity of the cellular membrane. Effective viscoelastic parameters characterizing red blood cells with different stiffnesses (normal and glutaraldehyde-fixed) are determined. It is shown that the photo-induced effects caused by laser trapping at the power level used in the experiments are negligible giving the possibility to use the offered technique for dynamic monitoring of soft materials viscoelastic properties.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Evgeny V. Lyubin ; Maria D. Khokhlova ; Maria N. Skryabina and Andrey A. Fedyanin
"Cellular viscoelasticity probed by active rheology in optical tweezers", J. Biomed. Opt. 17(10), 101510 (Jun 08, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.10.101510


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