Research Papers: Imaging

Structural alterations in rat liver proteins due to streptozotocin-induced diabetes and the recovery effect of selenium: Fourier transform infrared microspectroscopy and neural network study

[+] Author Affiliations
Ozlem Bozkurt, Feride Severcan

Middle East Technical University, Department of Biological Sciences, 06800 Ankara, Turkey

Sevgi Haman Bayari

Hacettepe University, Department of Physics Education, Faculty of Education, Beytepe 06800 Ankara, Turkey

Mete Severcan

Middle East Technical University, Department of Electrical and Electronics Engineering, 06800 Ankara, Turkey

Christoph Krafft

Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany

Jürgen Popp

Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany

University Jena, Institute of Physical Chemistry, Helmholtzweg 4, 07743 Jena, Germany

J. Biomed. Opt. 17(7), 076023 (Jul 17, 2012). doi:10.1117/1.JBO.17.7.076023
History: Received February 13, 2012; Revised June 21, 2012; Accepted June 22, 2012
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Abstract.  The relation between protein structural alterations and tissue dysfunction is a major concern as protein fibrillation and/or aggregation due to structural alterations has been reported in many disease states. In the current study, Fourier transform infrared microspectroscopic imaging has been used to investigate diabetes-induced changes on protein secondary structure and macromolecular content in streptozotocin-induced diabetic rat liver. Protein secondary structural alterations were predicted using neural network approach utilizing the amide I region. Moreover, the role of selenium in the recovery of diabetes-induced alterations on macromolecular content and protein secondary structure was also studied. The results revealed that diabetes induced a decrease in lipid to protein and glycogen to protein ratios in diabetic livers. Significant alterations in protein secondary structure were observed with a decrease in α-helical and an increase in β-sheet content. Both doses of selenium restored diabetes-induced changes in lipid to protein and glycogen to protein ratios. However, low-dose selenium supplementation was not sufficient to recover the effects of diabetes on protein secondary structure, while a higher dose of selenium fully restored diabetes-induced alterations in protein structure.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Ozlem Bozkurt ; Sevgi Haman Bayari ; Mete Severcan ; Christoph Krafft ; Jürgen Popp, et al.
"Structural alterations in rat liver proteins due to streptozotocin-induced diabetes and the recovery effect of selenium: Fourier transform infrared microspectroscopy and neural network study", J. Biomed. Opt. 17(7), 076023 (Jul 17, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.076023


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