Research Papers: Therapeutic

In vitro investigation of efficient photodynamic therapy using a nonviral vector; hemagglutinating virus of Japan envelope

[+] Author Affiliations
Makoto Sakai, Naohiro Fujimoto, Katsunori Ishii

Osaka University, Graduate School of Engineering, Osaka 565-0871, Japan

Hiroyuki Nakamura

Gakushuin University, Department of Chemistry, Tokyo 171-8588, Japan

Yasufumi Kaneda

Osaka University, Graduate School of Medicine, Osaka 565-0871, Japan

Kunio Awazu

Osaka University, Graduate School of Engineering, Osaka 565-0871, Japan

Osaka University, The Center of Advanced Medical Engineering and Informatics, Osaka 565-0871, Japan

Osaka University, Graduate School of Frontier Biosciences, Osaka 565-0871, Japan

J. Biomed. Opt. 17(7), 078002 (Jul 17, 2012). doi:10.1117/1.JBO.17.7.078002
History: Received November 28, 2011; Revised June 7, 2012; Accepted June 18, 2012
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Abstract.  Photodynamic therapy (PDT) is a photochemical modality approved for cancer treatment. PDT has demonstrated efficacy in early stage lung cancer and esophageal cancer. The accumulation of photosensitizers in cancer cells is necessary to enhance the therapeutic benefits of PDT; however, photosensitizers have low uptake efficiency. To overcome this limitation, a drug delivery system, such as the hemagglutinating virus of Japan envelope (HVJ-E) vector, is required. In this study, the combination of PDT and HVJ-E was investigated for enhancing the efficacy of PDT. The photosensitizers that were evaluated included 5-aminolaevulinic acid (5-ALA), protoporphyrin IX (PPIX), and HVJ-PPIX. The uptake of the photosensitizers as increased twenty-fold with the addition of HVJ-E. The cytotoxicity of conventional 5-ALA was enhanced by the addition of HVJ-E vector. In conclusion, HVJ-E vector improved the uptake of photosensitizers and the PDT effect.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Makoto Sakai ; Naohiro Fujimoto ; Katsunori Ishii ; Hiroyuki Nakamura ; Yasufumi Kaneda, et al.
"In vitro investigation of efficient photodynamic therapy using a nonviral vector; hemagglutinating virus of Japan envelope", J. Biomed. Opt. 17(7), 078002 (Jul 17, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.078002


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