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Research Papers

Noninvasive time-dependent cytometry monitoring by digital holography

[+] Author Affiliations
Manuel Kemmler, Markus Fratz, Dominik Giel, Norbert Saum, Albrecht Brandenburg, Christian Hoffmann

Fraunhofer Institute for Physical Measurement Techniques, Heidenhofstrasse 8, 79110 Freiburg, Germany

J. Biomed. Opt. 12(6), 064002 (November 20, 2007). doi:10.1117/1.2804926
History: Received March 08, 2007; Revised June 14, 2007; Accepted June 16, 2007; Published November 20, 2007
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Using a digital holographic microscope setup, it is possible to measure dynamic volume changes in living cells. The cells were investigated time-dependently in transmission mode for different kinds of stimuli affecting their morphology. The measured phase shift was correlated to the cellular optical thickness, and then of the cell volume as well as the refractive index were calculated and interpreted. For the characterization of the digital holographic microscope setup, we have developed a transparent three-dimensional (3-D) reference chart that can be used as a lateral resolution chart and step-height resolution chart included in one substrate. For the monitoring of living cells, a biocompatible and autoclavable flow chamber was designed, which allows us to add, exchange, or dilute the fluid within the flow chamber. An integrated changeable coverslip enables inverse microscopic applications. Trypsinization, cell swelling and shrinking induced by osmolarity changes, and apoptosis served as model processes to elucidate the potential of the digital holographic microscopy (DHM).

© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Manuel Kemmler ; Markus Fratz ; Dominik Giel ; Norbert Saum ; Albrecht Brandenburg, et al.
"Noninvasive time-dependent cytometry monitoring by digital holography", J. Biomed. Opt. 12(6), 064002 (November 20, 2007). ; http://dx.doi.org/10.1117/1.2804926


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