Research Papers

Endotoxemia increases the clearance of mPEGylated 5000-MW quantum dots as revealed by multiphoton microvascular imaging

[+] Author Affiliations
Ryon M. Bateman, Kevin C. Hodgson, Kapil Kohli, Darryl Knight, Keith R. Walley

University of British Columbia, Critical Care Research Laboratories, James Hogg iCAPTURE Centre, St. Paul’s Hospital, Vancouver, British Colombia, Canada, V6Z 1Y6

J. Biomed. Opt. 12(6), 064005 (December 18, 2007). doi:10.1117/1.2822882
History: Received April 04, 2007; Revised July 20, 2007; Accepted August 28, 2007; Published December 18, 2007
Text Size: A A A

Imaging the microcirculation is becoming increasingly important in assessing life-threatening disease states. To address this issue in a highly light absorbing and light scattering tissue, we use laser scanning multiphoton microscopy and fluorescent 655-nm5000-MW methoxy-PEGylated quantum dots to image the functional microcirculation deep in mouse hind limb skeletal muscle. Using this approach, we are able to minimize in vivo background tissue autofluorescence and visualize complete 3-D microvascular units, including feeding arterioles, capillary networks, and collecting venules to depths of 150to200μm. In CD1 mice treated with lipopolysaccharide to model an endotoxemic response to bacterial infection, we find that these quantum dots accumulate at microvascular bifurcations and extravasate from the microcirculation in addition to accumulating in organs (liver, spleen, lung, and kidney). The quantum dots are cleared from the circulation with a first-order elimination rate constant seven times greater than under normal conditions, 1.6±0.06 compared to 0.23±0.05h1, P<0.05, thereby reducing the imaging time window. In vitro experiments using TNFalpha treated isolated leukocytes suggest that circulating monocytes (phagocytes) increased their nonspecific uptake of quantum dots when activated. In combination with multiphoton microscopy, quantum dots provide excellent in vivo imaging contrast of deep microvascular structures.

© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Ryon M. Bateman ; Kevin C. Hodgson ; Kapil Kohli ; Darryl Knight and Keith R. Walley
"Endotoxemia increases the clearance of mPEGylated 5000-MW quantum dots as revealed by multiphoton microvascular imaging", J. Biomed. Opt. 12(6), 064005 (December 18, 2007). ; http://dx.doi.org/10.1117/1.2822882


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.