Research Papers

In vivo imaging of elastic fibers using sulforhodamine B

[+] Author Affiliations
Clément Ricard

INSERM, U836, Grenoble Institut des Neurosciences, Grenoble, F-38042 France and Université Joseph Fourier, BP 53, Grenoble Cedex 9, F38041 France

Jean-Claude Vial, Julien Douady

CNRS UMR 5588, Laboratoire de Spectrométrie Physique, Saint Martin d’Hères, F-38042 France, and Université Joseph Fourier, BP 53, Grenoble Cedex 9, F-38041 France

Boudewijn van der Sanden

INSERM, U836, Grenoble Institut des Neurosciences, Grenoble, F-38042 France and Université Joseph Fourier, BP 53, Grenoble Cedex 9, F38041 France

J. Biomed. Opt. 12(6), 064017 (December 18, 2007). doi:10.1117/1.2821421
History: Received February 28, 2007; Revised July 04, 2007; Accepted August 03, 2007; Published December 18, 2007
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Until now, the imaging of elastic fibers was restricted to tissue sections using the endofluorescence properties of elastin or histological dyes. Methods to study their morphology in vivo and in situ have been lacking. We present and characterize a new application of a fluorescent dye for two-photon microscopy: sulforhodamine B (SRB), which is shown to specifically stain elastic fibers in vivo. SRB staining of elastic fibers is demonstrated to be better than using elastin endofluorescence for two-photon microscopy. Our imaging method of elastic fibers is shown to be suitable for simultaneous imaging with both other fluorescent intravital dyes and second-harmonic generation (SHG). We illustrate these findings with intravital imaging of elastic and collagen fibers in muscle epimysium and endomysium and in blood vessel walls. We expect SRB staining to become a key method to study elastic fibers in vivo.

© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Clément Ricard ; Jean-Claude Vial ; Julien Douady and Boudewijn van der Sanden
"In vivo imaging of elastic fibers using sulforhodamine B", J. Biomed. Opt. 12(6), 064017 (December 18, 2007). ; http://dx.doi.org/10.1117/1.2821421


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