Research Papers

Quantitative analysis of structural disorder in intervertebral disks using second harmonic generation imaging: comparison with morphometric analysis

[+] Author Affiliations
Karen M. Reiser

University of California, Davis, Department of Neurological Surgery, Davis, California 95616

Clayton Bratton

University of California, Davis, Department of Physics, Davis, California 95616

Diego R. Yankelevich, André Knoesen, Israel Rocha-Mendoza

University of California, Davis, Department of Electrical and Computer Engineering, Davis, California 95616

Jeffrey Lotz

University of California, Davis, Department of Orthopaedic Surgery, San Francisco, California 94143

J. Biomed. Opt. 12(6), 064019 (December 07, 2007). doi:10.1117/1.2812631
History: Received July 26, 2006; Revised June 07, 2007; Accepted June 29, 2007; Published December 07, 2007
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A novel signal processing algorithm for quantifying structural disorder in biological tissue using second harmonic generation (SHG) imaging is described. Both the magnitude and the pattern of disorder in collagenous tissues can be determined with this method. Mathematical models are used to determine the range of disordered states over which the algorithm can be used, because highly disordered biological samples do not generate second harmonic signals. The method is validated by measuring disorder in heated fascicles using SHG and showing that results are significantly correlated with morphometric determination. Applicability of the method to tissue pathology is demonstrated by analysis of a mouse model of intervertebral disk injury. Disks were subjected to tensile or compressive forces in vivo for one week. Structural disorder in the annulus fibrosus was measured by SHG scanning and by standard morphometric analysis. Values for disorder obtained by SHG scanning were significantly correlated with values obtained by morphometry (p<0.001). Quantitation of disorder using SHG offers significant advantages over morphometric determination. Data obtained in this study suggest that this method can be used to discriminate between reversible and irreversible tissue damage.

© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Karen M. Reiser ; Clayton Bratton ; Diego R. Yankelevich ; André Knoesen ; Israel Rocha-Mendoza, et al.
"Quantitative analysis of structural disorder in intervertebral disks using second harmonic generation imaging: comparison with morphometric analysis", J. Biomed. Opt. 12(6), 064019 (December 07, 2007). ; http://dx.doi.org/10.1117/1.2812631


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