Research Papers

Extended depth-of-focus microscopy via constrained deconvolution

[+] Author Affiliations
José-Angel Conchello

Oklahoma Medical Research Foundation, Molecular, Cell, and Developmental Biology Program, 825 Northeast 13th Street, Oklahoma City, Oklahoma 73104 and University of Oklahoma, Biomedical Engineering Program, College of Engineering, Norman, Oklahoma 73019

Michael E. Dresser

Oklahoma Medical Research Foundation, Molecular, Cell, and Developmental Biology Program, 825 Northeast 13th Street, Oklahoma City, Oklahoma 73104 and University of Oklahoma Health Sciences Center, Department of Cell Biology, Oklahoma City, Oklahoma 73190

J. Biomed. Opt. 12(6), 064026 (November 28, 2007). doi:10.1117/1.2812554
History: Received June 02, 2006; Revised July 19, 2007; Accepted July 19, 2007; Published November 28, 2007
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We derive a method for extended depth-of-focus imaging, i.e., a method to render a 2-D image of a thick specimen, such that all the structures within the specimen appear in focus and with greatly increased contrast. We acquire a single image while moving the specimen through focus. The resulting image, which is severely blurred and has very low contrast, is then deconvolved. In the deconvolved image, the entire depth of the specimen is in focus. Because the image is collected continuously while the specimen moves through focus, the acquisition time is short. Likewise, because the deconvolution is done in 2-D, it is done very quickly even with an iterative algorithm.

© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

José-Angel Conchello and Michael E. Dresser
"Extended depth-of-focus microscopy via constrained deconvolution", J. Biomed. Opt. 12(6), 064026 (November 28, 2007). ; http://dx.doi.org/10.1117/1.2812554


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