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Research Papers

Inactivation of viruses by laser-driven coherent excitations via impulsive stimulated Raman scattering process

[+] Author Affiliations
Kong-Thon Tsen

Arizona State University, Department of Physics, Tempe, Arizona 85287

Shaw-Wei D. Tsen, Chih-Long Chang

Johns Hopkins School of Medicine, Department of Pathology, Baltimore, Maryland 21231

Chien-Fu Hung

Johns Hopkins School of Medicine, Departments of Pathology and Oncology, Baltimore, Maryland 21231

T.-C. Wu

Johns Hopkins School of Medicine, Departments of Pathology, Oncology, Obstetrics and Gynecology, and Molecular Microbiology and Immunology, Baltimore, Maryland 21231

Juliann G. Kiang

Armed Forces Radiobiology Research Institute, Scientific Research Department and Uniformed Services University of the Health Sciences, Departments of Medicine and Pharmacology, Bethesda, Maryland 20889-5603

J. Biomed. Opt. 12(6), 064030 (April 16, 2007August 09, 2007August 13, 2007December 14, 2007). doi:10.1117/1.2821713
History: Received April 16, 2007; Revised August 09, 2007; Accepted August 13, 2007; Published December 14, 2007
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The inactivation of viruses such as M13 bacteriophages subject to excitations by a very low power visible femtosecond laser has been studied. Our experimental results show that for a visible femtosecond laser having λ=425nm and a pulse width of 100fs, the M13 bacteriophages are inactivated when the laser power density is greater than or equal to 49MWcm2. The medium lethal laser power density (LD50) is 51.94±0.14MWcm2. The functionality of M13 bacteriophages has been shown to be critically dependent on the pulse width as well as power density of the excitation laser. Our work demonstrates that by using a very low power visible femtosecond laser, it is plausible to inactivate viruses such as the M13 bacterio-phages through impulsive stimulated Raman scattering process. These experimental findings suggest a novel avenue of selectively inactivating microorganisms while leaving the sensitive materials unharmed by manipulating and controlling with femtosecond laser systems.

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© 2007 Society of Photo-Optical Instrumentation Engineers

Citation

Kong-Thon Tsen ; Shaw-Wei D. Tsen ; Chien-Fu Hung ; T.-C. Wu ; Juliann G. Kiang, et al.
"Inactivation of viruses by laser-driven coherent excitations via impulsive stimulated Raman scattering process", J. Biomed. Opt. 12(6), 064030 (April 16, 2007August 09, 2007August 13, 2007December 14, 2007). ; http://dx.doi.org/10.1117/1.2821713


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