Special Section on Photoacoustic Imaging and Sensing

In vivo photoacoustic imaging of mouse embryos

[+] Author Affiliations
Jan Laufer, Jon Cleary, Paul Beard

University College London, Department of Medical Physics and Bioengineering, Gower Street, London WC1E 6BT, United Kingdom

University College London, Centre for Advanced Biomedical Imaging, Department of Medicine and Institute of Child Health, Paul O’Gorman Building, 72 Huntley Street, London WC1E 6DD, United Kingdom

Francesca Norris, Peter Johnson, Mark Lythgoe

University College London, Centre for Advanced Biomedical Imaging, Department of Medicine and Institute of Child Health, Paul O’Gorman Building, 72 Huntley Street, London WC1E 6DD, United Kingdom

Edward Zhang, Bradley Treeby, Ben Cox

University College London, Department of Medical Physics and Bioengineering, Gower Street, London WC1E 6BT, United Kingdom

Pete Scambler

UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, United Kingdom

J. Biomed. Opt. 17(6), 061220 (May 18, 2012). doi:10.1117/1.JBO.17.6.061220
History: Received December 22, 2011; Revised February 29, 2012; Accepted March 7, 2012
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Abstract.  The ability to noninvasively image embryonic vascular anatomy in mouse models is an important requirement for characterizing the development of the normal cardiovascular system and malformations in the heart and vascular supply. Photoacoustic imaging, which can provide high resolution non invasive images of the vasculature based upon optical absorption by endogenous hemoglobin, is well suited to this application. In this study, photoacoustic images of mouse embryos were obtained ex vivo and in vivo. The images show intricate details of the embryonic vascular system to depths of up to 10 mm, which allowed whole embryos to be imaged in situ. To achieve this, an all-optical photoacoustic scanner and a novel time reversal image reconstruction algorithm, which provide deep tissue imaging capability while maintaining high spatial resolution and contrast were employed. This technology may find application as an imaging tool for preclinical embryo studies in developmental biology as well as more generally in preclinical and clinical medicine for studying pathologies characterized by changes in the vasculature.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Jan Laufer ; Francesca Norris ; Jon Cleary ; Edward Zhang ; Bradley Treeby, et al.
"In vivo photoacoustic imaging of mouse embryos", J. Biomed. Opt. 17(6), 061220 (May 18, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.6.061220


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