Special Section on Selected Topics in Biophotonics: Optical Coherence Tomography and Medical Imaging Using Diffuse Optics

Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system

[+] Author Affiliations
Weirong Mo, Daniel Rohrbach, Ulas Sunar

Roswell Park Cancer Institute, Department of Cell Stress Biology and PDT Center, Elm and Carlton Streets, Buffalo, New York, 14263

J. Biomed. Opt. 17(7), 071306 (May 16, 2012). doi:10.1117/1.JBO.17.7.071306
History: Received October 14, 2011; Revised February 20, 2012; Accepted March 5, 2012
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Abstract.  We report the tomographic imaging of a photodynamic therapy (PDT) photosensitizer, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) in vivo with time-domain fluorescence diffuse optical tomography (TD-FDOT). Simultaneous reconstruction of fluorescence yield and lifetime of HPPH was performed before and after PDT. The methodology was validated in phantom experiments, and depth-resolved in vivo imaging was achieved through simultaneous three-dimensional (3-D) mappings of fluorescence yield and lifetime contrasts. The tomographic images of a human head-and-neck xenograft in a mouse confirmed the preferential uptake and retention of HPPH by the tumor 24-h post-injection. HPPH-mediated PDT induced significant changes in fluorescence yield and lifetime. This pilot study demonstrates that TD-FDOT may be a good imaging modality for assessing photosensitizer distributions in deep tissue during PDT monitoring.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Weirong Mo ; Daniel Rohrbach and Ulas Sunar
"Imaging a photodynamic therapy photosensitizer in vivo with a time-gated fluorescence tomography system", J. Biomed. Opt. 17(7), 071306 (May 16, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.071306


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