Special Section on Selected Topics in Biophotonics: Optical Coherence Tomography and Medical Imaging Using Diffuse Optics

Three-dimensional simultaneous optical coherence tomography and confocal fluorescence microscopy for investigation of lung tissue

[+] Author Affiliations
Maria Gaertner, Peter Cimalla, Sven Meissner, Edmund Koch

Dresden University of Technology, Clinical Sensoring and Monitoring, Faculty of Medicine Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, Germany

Wolfgang M. Kuebler

Institute for Physiology and University of Toronto, Charité Berlin, Department of Surgery, Canada

J. Biomed. Opt. 17(7), 071310 (Jun 14, 2012). doi:10.1117/1.JBO.17.7.071310
History: Received October 3, 2011; Revised April 13, 2012; Accepted May 9, 2012
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Abstract.  Although several strategies exist for a minimal-invasive treatment of patients with lung failure, the mortality rate of acute respiratory distress syndrome still reaches 30% at minimum. This striking number indicates the necessity of understanding lung dynamics on an alveolar level. To investigate the dynamical behavior on a microscale, we used three-dimensional geometrical and functional imaging to observe tissue parameters including alveolar size and length of embedded elastic fibers during ventilation. We established a combined optical coherence tomography (OCT) and confocal fluorescence microscopy system that is able to monitor the distension of alveolar tissue and elastin fibers simultaneously within three dimensions. The OCT system can laterally resolve a 4.9 μm line pair feature and has an approximately 11 μm full-width-half-maximum axial resolution in air. confocal fluorescence microscopy visualizes molecular properties of the tissue with a resolution of 0.75 μm (laterally), and 5.9 μm (axially) via fluorescence detection of the dye sulforhodamine B specifically binding to elastin. For system evaluation, we used a mouse model in situ to perform lung distension by application of different constant pressure values within the physiological regime. Our method enables the investigation of alveolar dynamics by helping to reveal basic processes emerging during artificial ventilation and breathing.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Maria Gaertner ; Peter Cimalla ; Sven Meissner ; Wolfgang M. Kuebler and Edmund Koch
"Three-dimensional simultaneous optical coherence tomography and confocal fluorescence microscopy for investigation of lung tissue", J. Biomed. Opt. 17(7), 071310 (Jun 14, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.071310


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