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Research Papers: General

Morphological and chemical changes in dentin after using endodontic agents: Fourier transform Raman spectroscopy, energy-dispersive x-ray fluorescence spectrometry, and scanning electron microscopy study

[+] Author Affiliations
Fernanda Miori Pascon, Regina Maria Puppin-Rontani

University of Campinas, Pediatric Dentistry Department, Piracicaba Dental School, Av. Limeira, 901, Piracicaba, SP 13414-903, Brazil

Kamila Rosamilia Kantovitz

National Institute of Arthrits and Musculoskeletal and Skin Diseases, National Institutes of Health, 9000 Rockville Pike, Building 50, Room 4122. Bethesda, Maryland 20814

Luís Eduardo Silva Soares

Vale do Paraíba University (UNIVAP), Dental Materials and Operative Dentistry Department, School of Dentistry, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB. Av. Shishima Hifumi, 2911, São José dos Campos, SP 12244-000, Brazil

Ana Maria do Espírito Santo

Federal University of São Paulo (UNIFESP), Mathematical and Earth Sciences Department, Prof. Artur Riedel, 275; São Paulo, SP 09972-270, Brazil

Airton Abrahão Martin

Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB; Av. Shishima Hifumi, 2911. São José dos Campos, SP 12244-000, Brazil

J. Biomed. Opt. 17(7), 075008 (Jul 09, 2012). doi:10.1117/1.JBO.17.7.075008
History: Received January 8, 2012; Revised June 4, 2012; Accepted June 6, 2012
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Abstract.  We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber and divided by six groups (n=5): NT-no treatment; CHX-2% chlorhexidine; CHXE-2%chlorhexidine+17%EDTA; E-17% EDTA; SH5-5.25% NaOCl; SH5E-5.25%NaOCl+17%EDTA. The inorganic and organic content was analyzed by FT-Raman. μEDXRF examined calcium (Ca) and phosphorus (P) content as well as Ca/P ratio. Impressions of specimens were evaluated by SEM. Data were submitted to Kruskal-Wallis and Dunn tests (p<0.05). Differences were observed among groups for the 960cm1 peak. Ca and P content differences were significant (SH5>NT=SH5E>CHX>E>CHXE). CHXE and E presented the highest Ca/P ratio values compared to the other groups (p<0.05). The SEM images in the EDTA-treated groups had the highest number of open tubules. Erosion in the tubules was observed in CHX and SH5E groups. Endodontic agents change the inorganic and organic content of pulp chamber dentin. NaOCl used alone, or in association with EDTA, was the most effective agent considering chemical and morphological approaches.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Fernanda Miori Pascon ; Kamila Rosamilia Kantovitz ; Luís Eduardo Silva Soares ; Ana Maria do Espírito Santo ; Airton Abrahão Martin, et al.
"Morphological and chemical changes in dentin after using endodontic agents: Fourier transform Raman spectroscopy, energy-dispersive x-ray fluorescence spectrometry, and scanning electron microscopy study", J. Biomed. Opt. 17(7), 075008 (Jul 09, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.075008


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