Research Papers: Imaging

Polydimethylsiloxane embedded mouse aorta ex vivo perfusion model: proof-of-concept study focusing on atherosclerosis

[+] Author Affiliations
Xueya Wang, Marc P. Wolf, Rahel Bänziger Keel, Roman Lehner

University Hospital Basel, Nanomedicine Group, Clinic for Intensive Care, Petersgraben 4, CH-4031 Basel, Switzerland

Patrick R. Hunziker

University Hospital Basel, Nanomedicine Group, Clinic for Intensive Care, Petersgraben 4, CH-4031 Basel, Switzerland

CLINAM Foundation for Nanomedicine, Basel, Switzerland

J. Biomed. Opt. 17(7), 076006 (Jul 06, 2012). doi:10.1117/1.JBO.17.7.076006
History: Received February 6, 2012; Revised May 23, 2012; Accepted June 1, 2012
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Abstract.  Existing mouse artery ex vivo perfusion models have utilized arteries such as carotid, uterine, and mesenteric arteries, but not the aorta. However, the aorta is the principal vessel analyzed for atherosclerosis studies in vivo. We have devised a mouse aorta ex vivo perfusion model that can bridge this gap. Aortas from apoE(-/-) mice are embedded in a transparent, gas-permeable, and elastic polymer matrix [polydimethylsiloxane (PDMS)] and artificially perfused with cell culture medium under cell culture conditions. After 24 h of artificial ex vivo perfusion, no evidence of cellular apoptosis is detected. Utilizing a standard confocal microscope, it is possible to image specific receptor targeting of cells in atherosclerotic plaques during 24 h. Imaging motion artifacts are minimal due to the polymer matrix embedding. Re-embedding of the aorta enables tissue sectioning and immuno-histochemical analysis. The ex vivo data are validated by comparison with in vivo experiments. This model can save animal lives via production of multiple endpoints in a single experiment, is easy to apply, and enables straightforward comparability with pre-existing atherosclerosis in vivo data. It is suited to investigate atherosclerotic disease in particular and vascular biology in general.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Xueya Wang ; Marc P. Wolf ; Rahel Bänziger Keel ; Roman Lehner and Patrick R. Hunziker
"Polydimethylsiloxane embedded mouse aorta ex vivo perfusion model: proof-of-concept study focusing on atherosclerosis", J. Biomed. Opt. 17(7), 076006 (Jul 06, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.7.076006


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