In addition to wavenumber positions, we evaluated the band intensities of the following characteristic vibrations: (phenylalanine ring breathing), (-deformation), (-twisting), (amide III), and (amide I and -stretching), which can be used to characterize the protein-lipid composition of biological tissue:26,27 while the the first band at can be used as marker for proteins, the vibrations at and display biomaterial and lipid content, respectively. The two other bands at and can be assigned to heme and also to lipids and proteins. These five bands form a so-called metric28 and are used throughout this paper. This metric was chosen according to Mahadevan-Jansen et al.29 who discriminated between cervical epithelium, inflammation, metaplasia, low-grade and high grade lesions utilizing Raman bands at 1246, 1330, 1454, and that are similar to our metric. After calculating the band heights of these five Raman bands, a (two-sided) Student’s -test30,31 was performed between all combinations of groups (B, E, C, M, N) and for all band heights. The obtained -values were all smaller than and therefore the significance level was ***. Here the common definition of the significance levels (* ; ** ; *** ) was utilized. This result permits the training of a classification technique.