JBO Letters

Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror

[+] Author Affiliations
Junjie Yao, Lidai Wang, Joon-Mo Yang, Liang Gao, Konstantin I. Maslov, Lihong V. Wang

Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St. Louis, Missouri 63130

Chih-Hsien Huang, Jun Zou

Texas A&M University, Department of Electrical and Computer Engineering, College Station, Texas 77843

J. Biomed. Opt. 17(8), 080505 (Aug 14, 2012). doi:10.1117/1.JBO.17.8.080505
History: Received June 1, 2012; Revised July 19, 2012; Accepted July 24, 2012
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Abstract.  By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Junjie Yao ; Chih-Hsien Huang ; Lidai Wang ; Joon-Mo Yang ; Liang Gao, et al.
"Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror", J. Biomed. Opt. 17(8), 080505 (Aug 14, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.8.080505


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