Thick () ex vivo specimens of human epidermis, obtained as discarded tissue from Mohs surgeries, were imaged with a confocal microscope (Vivascope 2000, Lucid, Rochester, NY). The microscope uses an 830-nm diode laser for illumination and a combination of polygon and galvanometric mirrors to scan the focused spot within the sample. A water immersion objective lens (Photon Gear, Ontario, NY) was used, with an adjustable iris allowing the effective NA to be selected between 0.5 and 0.9. With this objective and the Vivascope’s internal magnification and scan timing, a field of view of approximately () was imaged; this is magnification from object plane to pinhole plane. The optical sectioning was measured by translating a planar glass interface through focus with a calibrated piezo-stage (Melles Griot Nanomotion II) and recording the detected signal. The theoretical lateral resolution (0.4 to 0.6 μm) for all investigated combinations of pinholes sizes and NAs was less than or equal to the pixelation (0.6 μm) within the image. For each experimental setting, the laser power was adjusted, and the video signal’s histogram monitored to ensure complete use of the detection channel’s dynamic range with no saturated pixels. Three successive video frames were averaged to remove the effect of the microscope’s asynchronous timing jitter.