Research Papers: Imaging

Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function

[+] Author Affiliations
Timothy Kassis

Georgia Institute of Technology, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia

Georgia Institute of Technology, School of Electrical and Computer Engineering, Atlanta, Georgia

Alison B. Kohan, Patrick Tso

University of Cincinnati, Department of Pathology and Laboratory Medicine, Cincinnati, Ohio

Michael J. Weiler

Georgia Institute of Technology, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia

Georgia Institute of Technology, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia

Matthew E. Nipper

Georgia Institute of Technology, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia

Georgia Institute of Technology, George W. Woodruff School of Mechanical Engineering, Atlanta, Georgia

Rachel Cornelius

Georgia Institute of Technology, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia

J. Brandon Dixon

Georgia Institute of Technology, Parker H. Petit Institute for Bioengineering and Bioscience, Atlanta, Georgia

Georgia Institute of Technology, George W. Woodruff School of Mechanical Engineering, Atlanta, Georgia

Georgia Institute of Technology, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia

J. Biomed. Opt. 17(8), 086005 (Aug 07, 2012). doi:10.1117/1.JBO.17.8.086005
History: Received May 16, 2012; Revised July 9, 2012; Accepted July 11, 2012
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Abstract.  Nearly all dietary lipids are transported from the intestine to venous circulation through the lymphatic system, yet the mechanisms that regulate this process remain unclear. Elucidating the mechanisms involved in the functional response of lymphatics to changes in lipid load would provide valuable insight into recent implications of lymphatic dysfunction in lipid related diseases. Therefore, we sought to develop an in situ imaging system to quantify and correlate lymphatic function as it relates to lipid transport. The imaging platform provides the capability of dual-channel imaging of both high-speed bright-field video and fluorescence simultaneously. Utilizing post-acquisition image processing algorithms, we can quantify correlations between vessel pump function, lymph flow, and lipid concentration of mesenteric lymphatic vessels in situ. All image analysis is automated with customized LabVIEW virtual instruments; local flow is measured through lymphocyte velocity tracking, vessel contraction through measurements of the vessel wall displacement, and lipid uptake through fluorescence intensity tracking of an orally administered fluorescently labelled fatty acid analogue, BODIPY FL C16. This system will prove to be an invaluable tool for scientists studying intestinal lymphatic function in health and disease, and those investigating strategies for targeting the lymphatics with orally delivered drugs to avoid first pass metabolism.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Timothy Kassis ; Alison B. Kohan ; Michael J. Weiler ; Matthew E. Nipper ; Rachel Cornelius, et al.
"Dual-channel in-situ optical imaging system for quantifying lipid uptake and lymphatic pump function", J. Biomed. Opt. 17(8), 086005 (Aug 07, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.8.086005


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