Research Papers: Imaging

Förster resonance energy transfer photoacoustic microscopy

[+] Author Affiliations
Yu Wang, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, 1 Brookings Drive, St. Louis, Missouri, 63130

J. Biomed. Opt. 17(8), 086007 (Aug 08, 2012). doi:10.1117/1.JBO.17.8.086007
History: Received June 12, 2012; Revised July 10, 2012; Accepted July 12, 2012
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Abstract.  Förster, or fluorescence, resonance energy transfer (FRET) provides fluorescence signals sensitive to intra- and inter-molecular distances in the 1 to 10 nm range. Widely applied in the fluorescence imaging environment, FRET enables visualization of physicochemical processes in molecular interactions and conformations. In this paper, we report photoacoustic imaging of FRET, based on nonradiative decay that produces heat and subsequent acoustic waves. Estimates of the energy transfer efficiency by photoacoustic microscopy were compared to those obtained by fluorescence confocal microscopy. The experimental results in tissue phantoms show that photoacoustic microscopy is capable of FRET imaging with an enhanced penetration depth. Through its ability to three-dimensionally image tissue with scalable resolution, photoacoustic microscopy could be a beneficial biomedical tool to broaden the in vivo application of FRET.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Yu Wang and Lihong V. Wang
"Förster resonance energy transfer photoacoustic microscopy", J. Biomed. Opt. 17(8), 086007 (Aug 08, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.8.086007


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