Special Section on Optical Diagnostic and Biophotonic Methods from Bench to Bedside

Assessment of a custom-built Raman spectroscopic probe for diagnosis of early oesophageal neoplasia

[+] Author Affiliations
L. Max Almond

Gloucestershire Hospitals NHS Trust, Biophotonics Research Unit, Gloucester, Gloucestershire GL1 3NN, United Kingdom

Gloucestershire Hospitals NHS Trust, Department of Oesophagogastric Surgery, Gloucester, Gloucestershire GL1 3NN, United Kingdom

Jo Hutchings, Catherine Kendall, Gavin R. Lloyd

Gloucestershire Hospitals NHS Trust, Biophotonics Research Unit, Gloucester, Gloucestershire GL1 3NN, United Kingdom

John C. C. Day, Oliver A. C. Stevens

University of Bristol, Interface Analysis Centre, United Kingdom

Neil A. Shepherd

Gloucestershire Hospitals NHS Trust, Department of Histopathology, Gloucester, Gloucestershire GL1 3NN, United Kingdom

Hugh Barr

Gloucestershire Hospitals NHS Trust, Department of Oesophagogastric Surgery, Gloucester, Gloucestershire GL1 3NN, United Kingdom

Nick Stone

Gloucestershire Hospitals NHS Trust, Biophotonics Research Unit, Gloucester, Gloucestershire GL1 3NN, United Kingdom

University of Exeter, School of Physics, United Kingdom

J. Biomed. Opt. 17(8), 081421 (Sep 03, 2012). doi:10.1117/1.JBO.17.8.081421
History: Received December 16, 2011; Revised July 24, 2012; Accepted August 2, 2012
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Abstract.  We evaluate the potential of a custom-built fiber-optic Raman probe, suitable for in vivo use, to differentiate between benign, metaplastic (Barrett’s oesophagus), and neoplastic (dysplastic and malignant) oesophageal tissue ex vivo on short timescales. We measured 337 Raman spectra (λex=830nm; Pex=60mW; t=1s) using a confocal probe from fresh (298) and snap-frozen (39) oesophageal tissue collected during surgery or endoscopy from 28 patients. Spectra were correlated with histopathology and used to construct a multivariate classification model which was tested using leave one tissue site out cross-validation in order to evaluate the diagnostic accuracy of the probe system. The Raman probe system was able to differentiate, when tested with leave one site out cross-validation, between normal squamous oesophagus, Barrett’s oesophagus and neoplasia with sensitivities of (838% to 6%) and specificities of (89% to 99%). Analysis of a two group model to differentiate Barrett’s oesophagus and neoplasia demonstrated a sensitivity of 88% and a specificity of 87% for classification of neoplastic disease. This fiber-optic Raman system can provide rapid, objective, and accurate diagnosis of oesophageal pathology ex vivo. The confocal design of this probe enables superficial mucosal abnormalities (metaplasia and dysplasia) to be classified in clinically applicable timescales paving the way for an in vivo trial.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

L. Max Almond ; Jo Hutchings ; Catherine Kendall ; John C. C. Day ; Oliver A. C. Stevens, et al.
"Assessment of a custom-built Raman spectroscopic probe for diagnosis of early oesophageal neoplasia", J. Biomed. Opt. 17(8), 081421 (Sep 03, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.8.081421


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