Research Papers: Imaging

Dynamic contrast-enhanced optical imaging of in vivo organ function

[+] Author Affiliations
Cyrus B. Amoozegar, Tracy Wang, Matthew B. Bouchard, Addason F. H. McCaslin, Elizabeth M. C. Hillman

Columbia University, Departments of Biomedical Engineering and Radiology, Laboratory for Functional Optical Imaging, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, New York 10027

William S. Blaner

Columbia University, Department of Medicine, 630 W. 168th Street, New York, New York 10032

Richard M. Levenson

University of California, Department of Pathology & Laboratory Medicine, Davis Medical Center, PATH Building, 4400 V Street, Sacramento, California 95817

J. Biomed. Opt. 17(9), 096003 (Sep 06, 2012). doi:10.1117/1.JBO.17.9.096003
History: Received May 2, 2012; Revised August 8, 2012; Accepted August 9, 2012
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Abstract.  Conventional approaches to optical small animal molecular imaging suffer from poor resolution, limited sensitivity, and unreliable quantitation, often reducing their utility in practice. We previously demonstrated that the in vivo dynamics of an injected contrast agent could be exploited to provide high-contrast anatomical registration, owing to the temporal differences in each organ’s response to the circulating fluorophore. This study extends this approach to explore whether dynamic contrast-enhanced optical imaging (DyCE) can allow noninvasive, in vivo assessment of organ function by quantifying the differing cellular uptake or wash-out dynamics of an agent in healthy and damaged organs. Specifically, we used DyCE to visualize and measure the organ-specific uptake dynamics of indocyanine green before and after induction of transient liver damage. DyCE imaging was performed longitudinally over nine days, and blood samples collected at each imaging session were analyzed for alanine aminotransferase (ALT), a liver enzyme assessed clinically as a measure of liver damage. We show that changes in DyCE-derived dynamics of liver and kidney dye uptake caused by liver damage correlate linearly with ALT concentrations, with an r2 value of 0.91. Our results demonstrate that DyCE can provide quantitative, in vivo, longitudinal measures of organ function with inexpensive and simple data acquisition.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Cyrus B. Amoozegar ; Tracy Wang ; Matthew B. Bouchard ; Addason F. H. McCaslin ; William S. Blaner, et al.
"Dynamic contrast-enhanced optical imaging of in vivo organ function", J. Biomed. Opt. 17(9), 096003 (Sep 06, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.9.096003


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