Research Papers: Imaging

Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration

[+] Author Affiliations
Pu Wang, Han-Wei Wang

Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907

Ping Wang

Purdue University, Department of Chemistry, West Lafayette, Indiana 47907

Ji-Xin Cheng

Purdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907

Purdue University, Department of Chemistry, West Lafayette, Indiana 47907

J. Biomed. Opt. 17(9), 096010 (Sep 14, 2012). doi:10.1117/1.JBO.17.9.096010
History: Received February 10, 2012; Revised August 3, 2012; Accepted August 21, 2012
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Abstract.  Photoacoustic microscopy using vibrational overtone absorption as a contrast mechanism allows bond-selective imaging of deep tissues. Due to the spectral similarity of molecules in the region of overtone vibration, it is difficult to interrogate chemical components using photoacoustic signal at single excitation wavelength. Here we demonstrate that lipids and collagen, two critical markers for many kinds of diseases, can be distinguished by multispectral photoacoustic imaging of the first overtone of C-H bond. A phantom consisting of rat-tail tendon and fat was constructed to demonstrate this technique. Wavelengths between 1650 and 1850 nm were scanned to excite both the first overtone and combination bands of C-H bonds. B-scan multispectral photoacoustic images, in which each pixel contains a spectrum, were analyzed by a multivariate curve resolution-alternating least squares algorithm to recover the spatial distribution of collagen and lipids in the phantom.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Pu Wang ; Ping Wang ; Han-Wei Wang and Ji-Xin Cheng
"Mapping lipid and collagen by multispectral photoacoustic imaging of chemical bond vibration", J. Biomed. Opt. 17(9), 096010 (Sep 14, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.9.096010


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