Research Papers: Sensing

Noninvasive fluorescence excitation spectroscopy for the diagnosis of oral neoplasia in vivo

[+] Author Affiliations
Jeyasingh Ebenezar, Singaravelu Ganesan, Prakasarao Aruna

Anna University, Department of Medical Physics, Chennai 600025, India

Radhakrishnan Muralinaidu, Kannan Renganathan, Thillai Rajasekaran Saraswathy

Ragas Dental College, Department of Oral & Maxillofacial Pathology, Chennai 600096, India

J. Biomed. Opt. 17(9), 097007 (Sep 21, 2012). doi:10.1117/1.JBO.17.9.097007
History: Received May 18, 2012; Revised August 17, 2012; Accepted August 23, 2012
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Abstract.  Fluorescence excitation spectroscopy (FES) is an emerging approach to cancer detection. The goal of this pilot study is to evaluate the diagnostic potential of FES technique for the detection and characterization of normal and cancerous oral lesions in vivo. Fluorescence excitation (FE) spectra from oral mucosa were recorded in the spectral range of 340 to 600 nm at 635 nm emission using a fiberoptic probe spectrofluorometer to obtain spectra from the buccal mucosa of 30 sites of 15 healthy volunteers and 15 sites of 10 cancerous patients. Significant FE spectral differences were observed between normal and well differentiated squamous cell carcinoma (WDSCC) oral lesions. The FE spectra of healthy volunteers consists of a broad emission band around 440 to 470 nm, whereas in WDSCC lesions, a new primary peak was seen at 410 nm with secondary peaks observed at 505, 540, and 580 nm due to the accumulation of porphyrins in oral lesions. The FE spectral bands of the WDSCC lesions resemble the typical absorption spectra of a porphyrin. Three potential ratios (I410/I505, I410/I540, and I410/I580) were calculated from the FE spectra and used as input variables for a stepwise linear discriminant analysis (SLDA) for normal and WDSCC groups. Leave-one-out (LOO) method of cross-validation was performed to check the reliability on spectral data for tissue characterization. The diagnostic sensitivity and specificity were determined for normal and WDSCC lesions from the scatter plot of the discriminant function scores. It was observed that diagnostic algorithm based on discriminant function scores obtained by SLDA–LOO method was able to distinguish WDSCC from normal lesions with a sensitivity of 100% and specificity of 100%. Results of the pilot study demonstrate that the FE spectral changes due to porphyrin have a good diagnostic potential; therefore, porphyrin can be used as a native tumor marker.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Jeyasingh Ebenezar ; Singaravelu Ganesan ; Prakasarao Aruna ; Radhakrishnan Muralinaidu ; Kannan Renganathan, et al.
"Noninvasive fluorescence excitation spectroscopy for the diagnosis of oral neoplasia in vivo", J. Biomed. Opt. 17(9), 097007 (Sep 21, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.9.097007


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