Research Papers: Imaging

Video-rate functional photoacoustic microscopy at depths

[+] Author Affiliations
Lidai Wang, Konstantin Maslov, Wenxin Xing, Alejandro Garcia-Uribe, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130-4899

J. Biomed. Opt. 17(10), 106007 (Oct 01, 2012). doi:10.1117/1.JBO.17.10.106007
History: Received June 21, 2012; Revised September 7, 2012; Accepted September 10, 2012
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Abstract.  We report the development of functional photoacoustic microscopy capable of video-rate high-resolution in vivo imaging in deep tissue. A lightweight photoacoustic probe is made of a single-element broadband ultrasound transducer, a compact photoacoustic beam combiner, and a bright-field light delivery system. Focused broadband ultrasound detection provides a 44-μm lateral resolution and a 28-μm axial resolution based on the envelope (a 15-μm axial resolution based on the raw RF signal). Due to the efficient bright-field light delivery, the system can image as deep as 4.8 mm in vivo using low excitation pulse energy (28 μJ per pulse, 0.35mJ/cm2 on the skin surface). The photoacoustic probe is mounted on a fast-scanning voice-coil scanner to acquire 40 two-dimensional (2-D) B-scan images per second over a 9-mm range. High-resolution anatomical imaging is demonstrated in the mouse ear and brain. Via fast dual-wavelength switching, oxygen dynamics of mouse cardio-vasculature is imaged in realtime as well.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Lidai Wang ; Konstantin Maslov ; Wenxin Xing ; Alejandro Garcia-Uribe and Lihong V. Wang
"Video-rate functional photoacoustic microscopy at depths", J. Biomed. Opt. 17(10), 106007 (Oct 01, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.10.106007


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