Research Papers: Sensing

Discrimination of selected species of pathogenic bacteria using near-infrared Raman spectroscopy and principal components analysis

[+] Author Affiliations
Fernanda SantAna de Siqueira e Oliveira

Universidade Paulista—UNIP, Instituto de Ciências e Saúde, Rod. Pres. Dutra, km 157, São José dos Campos, SP, Brazil 12240-420

Hector Enrique Giana

Laboratório Oswaldo Cruz, Pça. Cândida Maria Sawaya Giana, 128, São José dos Campos, SP, Brazil 12243-003

Landulfo Silveira

Universidade Camilo Castelo Branco—UNICASTELO, Biomedical Engineering Institute, Parque Tecnológico de São José dos Campos, Rod. Pres. Dutra, km 138, São José dos Campos, SP, Brazil 12247-004

J. Biomed. Opt. 17(10), 107004 (Oct 09, 2012). doi:10.1117/1.JBO.17.10.107004
History: Received April 25, 2012; Revised September 5, 2012; Accepted September 11, 2012
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Abstract.  A method, based on Raman spectroscopy, for identification of different microorganisms involved in bacterial urinary tract infections has been proposed. Spectra were collected from different bacterial colonies (Gram-negative: Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterobacter cloacae, and Gram-positive: Staphylococcus aureus and Enterococcus spp.), grown on culture medium (agar), using a Raman spectrometer with a fiber Raman probe (830 nm). Colonies were scraped from the agar surface and placed on an aluminum foil for Raman measurements. After preprocessing, spectra were submitted to a principal component analysis and Mahalanobis distance (PCA/MD) discrimination algorithm. We found that the mean Raman spectra of different bacterial species show similar bands, and S. aureus was well characterized by strong bands related to carotenoids. PCA/MD could discriminate Gram-positive bacteria with sensitivity and specificity of 100% and Gram-negative bacteria with sensitivity ranging from 58 to 88% and specificity ranging from 87% to 99%.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Fernanda SantAna de Siqueira e Oliveira ; Hector Enrique Giana and Landulfo Silveira, Jr.
"Discrimination of selected species of pathogenic bacteria using near-infrared Raman spectroscopy and principal components analysis", J. Biomed. Opt. 17(10), 107004 (Oct 09, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.10.107004


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