Research Papers: Imaging

In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma

[+] Author Affiliations
Yang Sun, Hongtao Xie, Jing Liu, Matthew Lam, Feifei Zhou, Julien Bec, Diego R. Yankelevich, Laura Marcu

University of California, Davis, Department of Biomedical Engineering, 451 Health Sciences Drive, Davis, California 95616

Abhijit J. Chaudhari, Wayne L. Monsky

University of California, Davis, School of Medicine, Department of Radiology, 4860 Y Street, Sacramento, California 95817

Allison Dobbie, Steven L. Tinling, D. Gregory Farwell

University of California, Davis, Department of Head and Neck Oncology, Skull Base Surgery, 2521 Stockton Boulevard, Suite 7200, Sacramento, California 95817

Regina F. Gandour-Edwards

University of California, Davis, Department of Pathology & Laboratory Medicine, 4400 V Street, Sacramento, California 95817

J. Biomed. Opt. 17(11), 116003 (Nov 01, 2012). doi:10.1117/1.JBO.17.11.116003
History: Received July 6, 2012; Revised September 19, 2012; Accepted September 24, 2012
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Abstract.  Tissue diagnostic features generated by a bimodal technique integrating scanning time-resolved fluorescence spectroscopy (TRFS) and ultrasonic backscatter microscopy (UBM) are investigated in an in vivo hamster oral carcinoma model. Tissue fluorescence is excited by a pulsed nitrogen laser and spectrally and temporally resolved using a set of filters/dichroic mirrors and a fast digitizer, respectively. A 41-MHz focused transducer (37-μm axial, 65-μm lateral resolution) is used for UBM scanning. Representative lesions of the different stages of carcinogenesis show that fluorescence characteristics complement ultrasonic features, and both correlate with histological findings. These results demonstrate that TRFS-UBM provide a wealth of co-registered, complementary data concerning tissue composition and structure as it relates to disease status. The direct co-registration of the TRFS data (sensitive to surface molecular changes) with the UBM data (sensitive to cross-sectional structural changes and depth of tumor invasion) is expected to play an important role in pre-operative diagnosis and intra-operative determination of tumor margins.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Yang Sun ; Hongtao Xie ; Jing Liu ; Matthew Lam ; Abhijit J. Chaudhari, et al.
"In vivo validation of a bimodal technique combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy for diagnosis of oral carcinoma", J. Biomed. Opt. 17(11), 116003 (Nov 01, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.116003


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