Research Papers: Imaging

Assessment of the effects of ultrasound-mediated glucose on permeability of normal, benign, and cancerous human lung tissues with the Fourier-domain optical coherence tomography

[+] Author Affiliations
Huajiang Wei, Zhouyi Guo, Xiao Guo

South China Normal University, College of Biophotonics, MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangzhou 510631, Guangdong Province, China

Guoyong Wu

Sun Yat-Sen University, First Affiliated Hospital, Department of Surgery, Guangzhou 510080, Guangdong Province, China

Hongqin Yang, Shusen Xie

Fujian Normal University, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education of China, Fuzhou 350007, Fujian, China

Yonghong He

Tsinghua University, Graduate School at Shenzhen, Shenzhen 518055, Guangdong, China

J. Biomed. Opt. 17(11), 116006 (Nov 01, 2012). doi:10.1117/1.JBO.17.11.116006
History: Received May 22, 2012; Revised October 2, 2012; Accepted October 3, 2012
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Abstract.  The objective of this study was to evaluate the effects of ultrasound-mediated analyte diffusion on permeability of normal, benign, and cancerous human lung tissue in vitro and to find more effective sonophoretic (SP) delivery in combination with the optical clearing agents (OCAs) method to distinguish normal and diseased lung tissues. The permeability coefficients of SP in combination with OCAs diffusion in lung tissue were measured with Fourier-domain optical coherence tomography (FD-OCT). 30% glucose and SP with a frequency of 1 MHz and an intensity of 0.80W/cm2 over a 3 cm probe was simultaneously applied for 15 min. Experimental results show that the mean permeability coefficients of 30% glucose/SP were found to be (2.01±0.21)×105cm/s from normal lung (NL) tissue, (2.75±0.28)×105cm/s from lung benign granulomatosis (LBG) tissue, (4.53±0.49)×105cm/s from lung adenocarcinoma tumor (LAT) tissue, and (5.81±0.62)×105cm/s from lung squamous cell carcinoma (LSCC) tissue, respectively. The permeability coefficients of 30% glucose/SP increase approximately 36.8%, 125.4%, and 189.1% for the LBG, LAT, and LSCC tissue compared with that for the NL tissue, respectively. There were statistically significant differences in permeability coefficients of 30% glucose/SP between LBG and NL tissue (p<0.05), between LAT and NL tissue (p<0.05), and between LSCC and NL tissue (p<0.05). The results suggest that the OCT functional imaging technique to combine an ultrasound-OCAs combination method could become a powerful tool in early diagnosis and monitoring of changed microstructure of pathologic human lung tissue.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Huajiang Wei ; Guoyong Wu ; Zhouyi Guo ; Hongqin Yang ; Yonghong He, et al.
"Assessment of the effects of ultrasound-mediated glucose on permeability of normal, benign, and cancerous human lung tissues with the Fourier-domain optical coherence tomography", J. Biomed. Opt. 17(11), 116006 (Nov 01, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.116006


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