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Research Papers: General

Refractive index of solutions of human hemoglobin from the near-infrared to the ultraviolet range: Kramers-Kronig analysis

[+] Author Affiliations
Oleksiy Sydoruk

Imperial College London, Optical and Semiconductor Devices Group, Department of Electrical and Electronic Engineering, Exhibition Road, London SW7 2AZ, United Kingdom

Olga Zhernovaya

Saratov State University, International Research-Educational Center of Optical, Technologies for Industry and Medicine “Photonics,” 83 Astrakhanskaya Street, 410012 Saratov, Russia

Valery Tuchin

Saratov State University, International Research-Educational Center of Optical, Technologies for Industry and Medicine “Photonics,” 83 Astrakhanskaya Street, 410012 Saratov, Russia

University of Oulu, FI-90014, P.O. Box 4500, Oulu, Finland

Alexandre Douplik

Ryerson Technical University, Department of Physics, Faculty of Engineering, Architecture and Science, 50 Gould Street, Kerr Hall South, Toronto, ON, M5B2K3, Canada

Friedrich-Alexander University Erlangen-Nuremberg, Graduate School in Advanced Optical Technologies, Clinical Photonics Lab, SAOT Erlangen, Paul-Gordan-Strasse 6, 91052 Erlangen, Germany

J. Biomed. Opt. 17(11), 115002 (Nov 02, 2012). doi:10.1117/1.JBO.17.11.115002
History: Received June 8, 2012; Revised September 26, 2012; Accepted October 17, 2012
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Abstract.  Because direct measurements of the refractive index of hemoglobin over a large wavelength range are challenging, indirect methods deserve particular attention. Among them, the Kramers-Kronig relations are a powerful tool often used to derive the real part of a refractive index from its imaginary part. However, previous attempts to apply the relations to solutions of human hemoglobin have been somewhat controversial, resulting in disagreement between several studies. We show that this controversy can be resolved when careful attention is paid not only to the absorption of hemoglobin but also to the dispersion of the refractive index of the nonabsorbing solvent. We present a Kramers-Kroning analysis taking both contributions into account and compare the results with the data from several studies. Good agreement with experiments is found across the visible and parts of near-infrared and ultraviolet regions. These results reinstate the use of the Kramers-Kronig relations for hemoglobin solutions and provide an additional source of information about their refractive index.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Oleksiy Sydoruk ; Olga Zhernovaya ; Valery Tuchin and Alexandre Douplik
"Refractive index of solutions of human hemoglobin from the near-infrared to the ultraviolet range: Kramers-Kronig analysis", J. Biomed. Opt. 17(11), 115002 (Nov 02, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.115002


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