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Research Papers: Imaging

In vivo photoacoustic microscopy with 7.6-µm axial resolution using a commercial 125-MHz ultrasonic transducer

[+] Author Affiliations
Chi Zhang, Konstantin Maslov, Junjie Yao, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 17(11), 116016 (Nov 02, 2012). doi:10.1117/1.JBO.17.11.116016
History: Received July 13, 2012; Revised October 5, 2012; Accepted October 15, 2012
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Abstract.  Photoacoustic microscopy has achieved submicron lateral resolution, but its axial resolution is much lower. Here an axial resolution of 7.6 μm, the highest axial resolution validated by experimental data, has been achieved by using a commercial 125 MHz ultrasonic transducer for signal detection followed by the Wiener deconvolution for signal processing. Limited by the working distance, the high-frequency ultrasonic transducer can penetrate 1.2 mm into biological tissue from the ultrasound detection side. At this depth, the signal-to-noise ratio decreases by 11 dB, and the axial resolution degrades by 36%. The new system was demonstrated in imaging melanoma cells ex vivo and mouse ears in vivo.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Chi Zhang ; Konstantin Maslov ; Junjie Yao and Lihong V. Wang
"In vivo photoacoustic microscopy with 7.6-µm axial resolution using a commercial 125-MHz ultrasonic transducer", J. Biomed. Opt. 17(11), 116016 (Nov 02, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.116016


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