Research Papers: Imaging

Optical coherence tomography in vulvar intraepithelial neoplasia

[+] Author Affiliations
Ronni Wessels

Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Department of Surgery, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands

Daniel M. de Bruin

Academic Medical Center, Department of Biomedical Engineering and Physics, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands

Academic Medical Center, Department of Urology, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands

Dirk J. Faber

Academic Medical Center, Department of Biomedical Engineering and Physics, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands

Hester H. van Boven

Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Department of Pathology, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands

Andrew D. Vincent

Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Department of Biometrics, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands

Ton G. van Leeuwen

Academic Medical Center, Department of Biomedical Engineering and Physics, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands

University Twente, Biomedical Photonic Imaging Group, P.O. Box 217, 7500 AE, Enschede, The Netherlands

Marc van Beurden

Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Department of Gynaecology, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands

Theo J. M. Ruers

Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Department of Surgery, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands

University Twente, MIRA Institute, Nanobiophysics Group, P.O. Box 217, 7500 AE, Enschede, The Netherlands

J. Biomed. Opt. 17(11), 116022 (Nov 08, 2012). doi:10.1117/1.JBO.17.11.116022
History: Received July 11, 2012; Revised October 5, 2012; Accepted October 19, 2012
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Abstract.  Vulvar squamous cell carcinoma (VSCC) is a gynecological cancer with an incidence of two to three per 100,000 women. VSCC arises from vulvar intraepithelial neoplasia (VIN), which is diagnosed through painful punch biopsy. In this study, optical coherence tomography (OCT) is used to differentiate between normal and VIN tissue. We hypothesize that (a) epidermal layer thickness measured in OCT images is different in normal tissue and VIN, and (b) quantitative analysis of the attenuation coefficient (μoct) extracted from OCT data differentiates VIN from normal vulvar tissue. Twenty lesions from 16 patients are imaged with OCT. Directly after data acquisition, a biopsy is performed. Epidermal thickness is measured and values of μoct are extracted from 200 OCT scans of normal and VIN tissue. For both methods, statistical analysis is performed using Paired Mann–Whitney-test. Correlation between the two methods is tested using a Spearman-correlation test. Both epidermal layer thickness as well as the μoct are different between normal vulvar tissue and VIN lesions (p<0.0001). Moreover, no correlation is found between the epidermal layer thickness and μoct. This study demonstrates that both the epidermal thickness and the attenuation coefficient of vulvar epithelial tissue containing VIN are different from that of normal vulvar tissue.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Ronni Wessels ; Daniel M. de Bruin ; Dirk J. Faber ; Hester H. van Boven ; Andrew D. Vincent, et al.
"Optical coherence tomography in vulvar intraepithelial neoplasia", J. Biomed. Opt. 17(11), 116022 (Nov 08, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.116022


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