Research Papers: Imaging

Human brain cancer studied by resonance Raman spectroscopy

[+] Author Affiliations
Yan Zhou

The Air Force General Hospital, PLA, No. 30 Fuchenglu, Haidian District, Beijing 100142, China

Cheng-Hui Liu, Yang Pu, Robert R. Alfano

The City College of the City University of New York, Institute of Ultrafast Spectroscopy and Lasers, Department of Physics, New York, New York 10031

Yi Sun

The City College of the City University of New York, Department of Electrical Engineering, New York, New York 10031

Susie Boydston-White

City University of New York, Borough of Manhattan Community College, Science Department, New York, New York 10007-1097

Yulong Liu

Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China

J. Biomed. Opt. 17(11), 116021 (Nov 15, 2012). doi:10.1117/1.JBO.17.11.116021
History: Received June 20, 2012; Revised October 22, 2012; Accepted October 22, 2012
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Abstract.  The resonance Raman (RR) spectra of six types of human brain tissues are examined using a confocal micro-Raman system with 532-nm excitation in vitro. Forty-three RR spectra from seven subjects are investigated. The spectral peaks from malignant meningioma, stage III (cancer), benign meningioma (benign), normal meningeal tissues (normal), glioblastoma multiforme grade IV (cancer), acoustic neuroma (benign), and pituitary adenoma (benign) are analyzed. Using a 532-nm excitation, the resonance-enhanced peak at 1548cm1 (amide II) is observed in all of the tissue specimens, but is not observed in the spectra collected using the nonresonance Raman system. An increase in the intensity ratio of 1587 to 1605cm1 is observed in the RR spectra collected from meningeal cancer tissue as compared with the spectra collected from the benign and normal meningeal tissue. The peak around 1732cm1 attributed to fatty acids (lipids) are diminished in the spectra collected from the meningeal cancer tumors as compared with the spectra from normal and benign tissues. The characteristic band of spectral peaks observed between 2800 and 3100cm1 are attributed to the vibrations of methyl (CH3) and methylene (CH2) groups. The ratio of the intensities of the spectral peaks of 2935 to 2880cm1 from the meningeal cancer tissues is found to be lower in comparison with that of the spectral peaks from normal, and benign tissues, which may be used as a distinct marker for distinguishing cancerous tissues from normal meningeal tissues. The statistical methods of principal component analysis and the support vector machine are used to analyze the RR spectral data collected from meningeal tissues, yielding a diagnostic sensitivity of 90.9% and specificity of 100% when two principal components are used.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Yan Zhou ; Cheng-Hui Liu ; Yi Sun ; Yang Pu ; Susie Boydston-White, et al.
"Human brain cancer studied by resonance Raman spectroscopy", J. Biomed. Opt. 17(11), 116021 (Nov 15, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.11.116021


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