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Special Section on Optical Methods of Imaging in the Skin

Switchable skin window induced by optical clearing method for dermal blood flow imaging

[+] Author Affiliations
Jing Wang, Rui Shi, Dan Zhu

Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan, China

Huazhong University of Science and Technology, Key Laboratory of Biomedical Photonics of Ministry of Education, Department of Biomedical Engineering, Wuhan, China

J. Biomed. Opt. 18(6), 061209 (Nov 15, 2012). doi:10.1117/1.JBO.18.6.061209
History: Received September 5, 2012; Revised October 19, 2012; Accepted October 22, 2012
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Abstract.  Optical imaging techniques have shown tremendous potential for assessing cutaneous microcirculation, but the imaging depth and contrast is limited by the strong scattering of skin. Current skin windows have to be fulfilled by surgical operation and suffer from some side effects. In this study, a switchable skin window was developed by topical application of an optical clearing agent (OCA) and saline on rat skin in vivo. The validity of the skin window was evaluated by the laser speckle contrast imaging technique, and the safety of OCA to the body was tested through histologic examinations. The results indicated that administration of OCA or saline on rat skin in vivo can open or close the window of skin repeatedly for three days. With the repair effect of hyaluronic acid and Vaseline, it is able to repeatedly visualize the dermal blood vessels and flow distribution. Long-term observation shows that there is no abnormal reflection in micro-structure, body weight, organ coefficients, histopathologic lesions, or toxic reactions compared with a control group. This switchable window will provide an effective tool not only for cutaneous microcirculation with laser speckle contrast imaging, but also for diagnosis and treatment of peripheral vascular diseases, including tumor research with various optical imaging techniques.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Jing Wang ; Rui Shi and Dan Zhu
"Switchable skin window induced by optical clearing method for dermal blood flow imaging", J. Biomed. Opt. 18(6), 061209 (Nov 15, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.6.061209


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